The Ohxyphog System is manufactured under EPA Establishment #: 83124-FL-00

Guardian Ozone Testing at Supermarket

On May 5,2020 the meat preparation room at an Orlando Supermarket was re-treated with the Ohxyphog system to provide enhanced disinfection of the all contact surfaces and a continued reduction of the bio-load within the prep room. The system was set up with the 2 ozone fogging stacks, spaced apart in the prep room. The localized A/C chiller for the prep room was shut down for the duration of the test, the cooling fans were turned off and a floor blower was used to provide stirring of the air and ozone fog in the room. The ozone concentration and Ct trends are provided in ​Figures 1 and 2​.

OhxyPhog Validation for Room Sanitation

Microbiology study at WSU Tree Fruit Extension

Development of a Practical Method for Using Ozone Gas as a Virus Decontaminating Agent

The anti-viral and anti-microbial properties of ozone have been well documented, although the mechanisms of action are not well understood, and several macromole-cular targets could be involved (Carpendale and Freeberg, 1991; Wells et al., 1991; Khadre and Yousef, 2002; Shin and Sobsey, 2003; Cataldo, 2006; Lin and Wu, 2006; Lin et al., 2007). Aqueous solutions of ozone are in use as disinfectants in many commercial situations, including waste water treatment, laundries, and food processing (Kim et al., 1999; Shin and Sobsey, 2003; Naitou and Takahara, 2006, 2008; Cardis et al., 2007), but the use of the gas on a commercial scale as a decontamination device has not been exploited. Ozone gas however has a number of potential advantages over other decontaminating gases and liquid chemical applications (McDonnell and Russell, 1999; Barker et al., 2004).

Inactivation of Norovirus by ozone gas in conditions relevant to healthcare

We evaluated the ability of ozone gas to inactivate Norovirus and its animal surrogate feline calicivirus (FCV) in dried samples placed at various locations within a hotel room, a cruise liner cabin and an office. Norovirus was measured by quantitative reverse transcriptase real-time polymerase chain reaction (QRT-PCR) assay, and FCV by a combination of QRT-PCR and virus infectivity assays. We were able to reduce the concentration of infectious FCV by a factor of more than 103 , and in some cases beyond detection, under optimal conditions of ozone exposure with less than an hour of total operation. QRT-PCR assays indicated similar decreases in both viral RNAs. Virus-containing samples dried onto hard surfaces (plastic, steel and glass), and soft surfaces such as fabric, cotton and carpet, were equally vulnerable to the treatment. Our results show that Norovirus can be inactivated by exposure to ozone gas from a portable commercial generator in settings such as hotel rooms, cruise ship cabins and healthcare facilities.

MRSA study

On March 13, 2007, a series of experiments (Experiment 4) were run in Orlando FL to determine whether or not the Vapex fog could kill MRSA - inoculated stainless steel coupons.

 

The previous owner of Vapex paid a company to inoculate his warehouse with a large amount of MRSA.The study showed that at a 4 ppm concentration for 15 minutes all of the MRSA had been killed.  MRSA is a bacteria, and viruses are much easier to kill than bacteria.

OhxyPhog Data from a Restaurant

Here are the bacti results for the Ohxyphog test:

 

Ozonation Rate: ~20 g/hour

Nozzle Water Flow: ~1.5 - 2.0 gph each

Calculated Ct:  ~134 ppmv-min

On May 5,2020 the meat preparation room at an Orlando Supermarket was re-treated with the Ohxyphog system to provide enhanced disinfection of the all contact surfaces and a continued reduction of the bio-load within the prep room.  The system was set up with the 2 ozone fogging stacks, spaced apart in the prep room.  The localized A/C chiller for the prep room was shut down for the duration of the test, the cooling fans were turned off and a floor blower was used to provide stirring of the air and ozone fog in the room. The ozone concentration and Ct trends are provided in Figures 1 and 2.

 

Before the test began, steel plates inoculated with Enterobacter were placed at various locations within the prep room.  Additionally, the plastic curtain, Figure 3, was swabbed before and after the ozone fog treatment for aerobic and Enterobacteriaceae bacteria.   When the Ohxyphog process was complete, the inoculated plates were removed, swabbed and sent to an independent lab for analysis.  The results of the inoculated plates and swabs are provided in Tables 1 and 2. 

OhxyPhog Disinfection of Orlando Supermarket Meat Prep Room

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Patent number: 8,029,726

Disclaimer:  The US EPA states that “pesticidal devices are subject to certain regulatory requirements under the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA), one of which is a prohibition of false or misleading labeling claims. Making false or misleading labeling claims about the safety or efficacy of a pesticidal device may result in penalties under FIFRA”.  OhxyPhog is manufactured by Guardian Manufacturing, Inc. under EPA Establishment # 83124-FL-00 and US Patent 8,029,726 “Disinfection Methods for Surface and Enclosed Spaces” and makes no claims or warrants that OhxyPhog can or will kill the COVID-19 coronavirus.  There are numerous scientific studies that show that ozone gas is effective in killing most viruses.  To the best of our knowledge, no scientific paper exists today as to the efficacy of any EPA registered disinfectant or EPA Pesticide Device in their ability to kill COVID19.  It’s our belief and the belief of the scientific community that ozone will be proven to kill COVID19 but until these studies are published, we cannot make any claim or warranty regarding the ability of OhxyPhog to kill COVID19.  As an EPA Pesticide Device Manufacturer, we have adequate data to show we can kill specific pathogens and have the necessary data to back up our claims (i.e. reference case studies). 

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