The Ohxyphog System is manufactured under EPA Establishment #: 83124-FL-00
Guardian Ozone Testing at Supermarket
On May 5,2020 the meat preparation room at an Orlando Supermarket was re-treated with the Ohxyphog system to provide enhanced disinfection of the all contact surfaces and a continued reduction of the bio-load within the prep room. The system was set up with the 2 ozone fogging stacks, spaced apart in the prep room. The localized A/C chiller for the prep room was shut down for the duration of the test, the cooling fans were turned off and a floor blower was used to provide stirring of the air and ozone fog in the room. The ozone concentration and Ct trends are provided in Figures 1 and 2.
OhxyPhog Validation for Room Sanitation
Microbiology study at WSU Tree Fruit Extension
Development of a Practical Method for Using Ozone Gas as a Virus Decontaminating Agent
The anti-viral and anti-microbial properties of ozone have been well documented, although the mechanisms of action are not well understood, and several macromole-cular targets could be involved (Carpendale and Freeberg, 1991; Wells et al., 1991; Khadre and Yousef, 2002; Shin and Sobsey, 2003; Cataldo, 2006; Lin and Wu, 2006; Lin et al., 2007). Aqueous solutions of ozone are in use as disinfectants in many commercial situations, including waste water treatment, laundries, and food processing (Kim et al., 1999; Shin and Sobsey, 2003; Naitou and Takahara, 2006, 2008; Cardis et al., 2007), but the use of the gas on a commercial scale as a decontamination device has not been exploited. Ozone gas however has a number of potential advantages over other decontaminating gases and liquid chemical applications (McDonnell and Russell, 1999; Barker et al., 2004).
Inactivation of Norovirus by ozone gas in conditions relevant to healthcare
We evaluated the ability of ozone gas to inactivate Norovirus and its animal surrogate feline calicivirus (FCV) in dried samples placed at various locations within a hotel room, a cruise liner cabin and an office. Norovirus was measured by quantitative reverse transcriptase real-time polymerase chain reaction (QRT-PCR) assay, and FCV by a combination of QRT-PCR and virus infectivity assays. We were able to reduce the concentration of infectious FCV by a factor of more than 103 , and in some cases beyond detection, under optimal conditions of ozone exposure with less than an hour of total operation. QRT-PCR assays indicated similar decreases in both viral RNAs. Virus-containing samples dried onto hard surfaces (plastic, steel and glass), and soft surfaces such as fabric, cotton and carpet, were equally vulnerable to the treatment. Our results show that Norovirus can be inactivated by exposure to ozone gas from a portable commercial generator in settings such as hotel rooms, cruise ship cabins and healthcare facilities.
MRSA study
On March 13, 2007, a series of experiments (Experiment 4) were run in Orlando FL to determine whether or not the Vapex fog could kill MRSA - inoculated stainless steel coupons.
The previous owner of Vapex paid a company to inoculate his warehouse with a large amount of MRSA.The study showed that at a 4 ppm concentration for 15 minutes all of the MRSA had been killed. MRSA is a bacteria, and viruses are much easier to kill than bacteria.
OhxyPhog Data from a Restaurant
Here are the bacti results for the Ohxyphog test:
Ozonation Rate: ~20 g/hour
Nozzle Water Flow: ~1.5 - 2.0 gph each
Calculated Ct: ~134 ppmv-min
On May 5,2020 the meat preparation room at an Orlando Supermarket was re-treated with the Ohxyphog system to provide enhanced disinfection of the all contact surfaces and a continued reduction of the bio-load within the prep room. The system was set up with the 2 ozone fogging stacks, spaced apart in the prep room. The localized A/C chiller for the prep room was shut down for the duration of the test, the cooling fans were turned off and a floor blower was used to provide stirring of the air and ozone fog in the room. The ozone concentration and Ct trends are provided in Figures 1 and 2.
Before the test began, steel plates inoculated with Enterobacter were placed at various locations within the prep room. Additionally, the plastic curtain, Figure 3, was swabbed before and after the ozone fog treatment for aerobic and Enterobacteriaceae bacteria. When the Ohxyphog process was complete, the inoculated plates were removed, swabbed and sent to an independent lab for analysis. The results of the inoculated plates and swabs are provided in Tables 1 and 2.
OhxyPhog Disinfection of Orlando Supermarket Meat Prep Room
